Tris(hydroxymethyl)aminomethane

☑ ʻAʻole hoʻohana nui ʻia ʻo Tris buffer ma ke ʻano he mea hoʻoheheʻe no nā ʻakika nucleic a me nā protein, akā he nui nā hoʻohana nui.Ua hoʻohana ʻia ʻo Tris no ka ulu ʻana o ke aniani protein ma lalo o nā kūlana pH like ʻole.
☑ Hiki ke hoʻohana ʻia ka ikaika ionic haʻahaʻa o Tris buffer e hana i ka fiber waena o ka lamin ma C. elegans.
☑ ʻO Tris kekahi o nā mea nui o ka protein electrophoresis buffer.
☑ Eia kekahi, ʻo Tris kahi mea waena no ka hoʻomākaukau ʻana i nā surfactants, nā mea hoʻolale vulcanization a me kekahi mau lāʻau lapaʻau.Hoʻohana ʻia ʻo Tris ma ke ʻano he titration standard.
☑Hoʻohana nui ʻia ʻo Trimethylaminomethane i ka metabolic acute acidemia.He paʻa alkaline a loaʻa kahi hopena pale maikaʻi i ka metabolic acidosis a me ka hopena o ka hana enzyme.
☑Hoʻohana pinepine ʻia ʻo Tris ma ke ʻano he mea hoʻopaʻa ola, a ʻo kona pH waiwai ʻo 6.8, 7.4, 8.0, 8.8.ʻO kāna kumu hoʻolālā, hoʻololi nui ka waiwai pH me ka mahana.Ma ka ʻōlelo maʻamau, e emi ana ka waiwai pH i ka 0.03 ke piʻi ka mahana i hoʻokahi degere.Hoʻohana nui ʻia ʻo Tris i ka hoʻomākaukau ʻana i ka hopena buffer i ka biochemistry a me nā hoʻokolohua molecular biology.No ka laʻana, ʻo ka TAE a me ka tbe buffers (hoʻohana ʻia no ka dissolution nucleic acid) i hoʻohana mau ʻia i nā hoʻokolohua biochemical pono iā Tris.No ka mea aia nā pūʻulu amino, hiki iā ia ke hana me nā aldehydes.
☑ He kumu nāwaliwali ʻo Tris, a ʻo kāna PKA he 8.1 ma ka lumi wela (25 ℃).Wahi a ke kumumanaʻo buffer, ʻo ka pae hoʻopono kūpono o Tris buffer ma waena o 7.0 a me 9.2.ʻO ka pH waiwai o Tris base aqueous solution ma kahi o 10.5.ʻO ka maʻamau, hoʻohui ʻia ka waika hydrochloric e hoʻoponopono i ka waiwai pH i ka waiwai i makemake ʻia e loaʻa ai ka hopena buffer me kēia waiwai pH.I ka manawa like, pono mākou e hoʻolohe i ka hopena o ka mahana ma ka pKa o Tris.No ka mea ʻo Tris buffer kahi hopena alkaline nāwaliwali, e hoʻopau ʻia ka DNA i loko o ia ʻano hopena e hoʻomaikaʻi ai i kona solubility.Hoʻohui pinepine ka poʻe i ka EDTA i loko o Tris hydrochloric acid buffer e hana i "Te buffer", i hoʻohana ʻia no ka hoʻopaʻa ʻana a me ka mālama ʻana o DNA.Inā hoʻololi ʻia ka ʻakika o ka hoʻoponopono ʻana i ka waiwai pH e ka acetic acid, loaʻa ka "Tae buffer" (Tris / acetate / EDTA), a loaʻa ka "tbe buffer" (Tris / borate / EDTA) ma ka hoʻololi ʻana iā ia me ka waikawa boric. .Hoʻohana mau ʻia kēia mau pale ʻelua i nā hoʻokolohua electrophoresis nucleic acid.